Pias1 is an e3 sumo ligase involved in various cellular processes. Jetpei transfection reagent from polyplus transfection. Polyplus transfection sa, a privatelyheld company specializing in the development of innovative solutions for transfection, today announces the launch of. Please feel free to browse the polyplus transfection database to find conditions for specific product, cell line or nucleic acid type. Polyplus transfection sa is a biotechnology company that develops, markets and sells innovative solutions for the in vivo, in vitro and ex vivo delivery of nucleic acids in research, bioproduction. Comparative study of polyethylenimines for transient gene. The optimal transfection time depends on the cell line, transfection reagent and nucleic acid used. Polyplus transfection launches a new transfection reagent. Click on the below button to be taken to the polyplustransfection website and search their transfection database.
Jan 18, 2012 strasbourg, france, january 18, 2012 polyplustransfection sa, specializing in the development of innovative solutions for transfection, today announces the launch of peipro, the next generation linear pei polyethylenimine transfection reagent for large scale production of proteins, antibodies and viral vectors. Polyplus transfection sa is a biotechnology company that develops, markets and sells innovative solutions for the in vivo, in vitro and ex vivo delivery of nucleic acids in research, bioproduction and therapeutics. Polyplus transfection sa is a biotechnology company researching, developing, manufacturing and marketing innovative solutions for scientists working in molecular and cell biology. May 01, 2004 we have also developed image analysis software that allows automatic detection of cell clusters, quantification of transfection efficiency and levels of expressionextinction of genes. Cell targeting is the result of binding of the mannose residues to the specific. Polyplus transfection products careforde scientific. Nih3t3 mouse fibroblastic cell line was transfected by pcmv6neocd31 recombinant expression vector using jetpei transfection reagent and transient expression of cd31 was confirmed by surface flow cytometry 48 hours after transfection figure 3c. Invitrogen lipofectamine transfection reagent protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. Vero and cos1 cell lines were transfected by different methods, both physical. If you find this doesnt work for your specific cell type, then you can look to our cell specific protocols below for further optimization.
Human embryonic kidney cell line hek293t and hek293t cells stably expressing. Our results indicated that 2271% of hek293ft cell line were easily transfected by the three commercial reagents used in this study figure 1. Polyplustransfection s vast portfolio of delivery solutions can be used for all types of applications, from research and process development, all the way to clinical trials. Transfection of plasmid dna encoding for the protein of interest in a given cell line can be used for both transient and stable protein production. Improved protein yields in cho and hek293 cells using a. Browse our cell transfection database to find the optimized conditions. Invitrogen lipofectamine transfection reagent protocols have been optimized for. We are focused on helping our customers be highly successful with their science. General information about the cell line or primary cell. Optimization of gene transfection in murine myeloma cell. The expression of the biosensor hyper in c2c12 myoblasts was achieved by transfection of the phypercyto vector, a plasmid into which the coding dna sequence of the biosensor hyper is incorporated, into c2c12 cells. Similarly, compared with human normal prostate epithelial cell line rwpe1, mir3 expression levels were significantly higher in pca cell lines pc3, 22rv1, lncap and du145 p cells showed the greatest mean mir3 expression relative to rwpe1 cells, they were chosen for all subsequent experiments.
Quantitative analysis of highly parallel transfection in. At polyplustransfection, we are proud to work with reagents that are extremely gentle to cells, maintaining an excellent cell viability and morphology throughout the whole duration of the experiments. Search in the publication database with polyplus transfection reagents or for optimal transfection conditions over 4000 publications, cell lines and primary. Cell transfection database over 4000 publications polyplus. Frequently asked questions about transfection polyplustransfection. Cell and tissue culture thermo fisher scientific us. Stable cell line generation is made possible by the use of positive selection markers such as g418, hygromycin b, puromycin resistance, etc selection markers can be delivered using the same plasmid that contains the gene of interest in cis, or on a separate plasmid in trans that needs to be cotransfected with the plasmid containing the gene of interest. Expression and functional analysis of the hydrogen. Readytorun transfection protocols eliminate the need for. Press release data bridge market research transfection technologies market 2020 rising demand with key players mirus bio llc, takara bio inc. Antibodies are also successfully delivered to hela cells and able to recognize their target protein inside the cytoplasm.
Fectopro will also produce up to three times more protein using half the dna required by other leading commercial transfection reagents. May 28, 2014 strasbourg, france, may 28, 2014 polyplustransfection sa, a privatelyheld company specializing in the development of innovative solutions for transfection, today announces the launch of fectoprotm, the latest optimised transfection reagent in their product portfolio. Transfection guide overview of transfection methods promega. Polyplustransfection sa is a biotechnology company that develops, markets and sells innovative solutions for the in vivo, in vitro and ex vivo delivery of nucleic acids in research, bioproduction and therapeutics. Qiagen protocols two types of protocol are provided in the transfect protocol database. Search for the optimal conditions for transfection of hundreds of mammalian cell lines. Several databases have been developed for exploring the link between. As always, qiagen cannot verify data supplied from outside sources. By providing a complete workflow for cell culture, we are now, more than ever in a position to supply all your cell culture needs and accelerate your cell biology research. Our team created the polyplustransfection database in which you can find the best transfection conditions for your cell lineas as well as product citations. Comprehensive characterization of circular rnas in.
Generalities about transfection polyplustransfection. Find optimized protocols, references and more in one easily searchable online. Quantitative analysis of highly parallel transfection in cell. Preparation of proper immunogen by cloning and stable. Stable cell line generation with transit transfection. Polyplus transfection licenses kempbio for mammalian. We performed a chemical transfection protocol based on the reagent jetpei polyplus transfection at a ratio of 6. Neon transfection system cell line data thermo fisher. In vitro technologies is committed to providing essential products to the scientific and medical community, especially during the current, quickly changing circumstances caused by the corona virus covid19 outbreak. Interferin from polyplus transfection,interferin is a newgeneration sirna transfection reagent that achieves over 90 % silencing efficiency at 1 nm sirna in a wide variety of adherent and primary cells with excellent cell viability. It is a linear watersoluble polymer, and is attracted to negatively charged phosphate groups on dna with which it forms stable aggregates.
As a starting point, use an amount of plasmid dna see column 4, dsrna or rnai vector dna see column 5, and lipofectamine 2000 see column 7 that falls around the midpoint of the recommended range, then optimize conditions for your cell line by varying. She provides an overview of solutions to deliver both guide rna. Dna can also be introduced into cells using viruses as a carrier. Located in the parc dinnovation at illkirch close to the city and university of strasbourg in eastern france, the company has been producing and selling its. May 06, 2009 jetpei, a transfection reagent from polyplus, is classified as a cationic polymer, with a positive charge on approximately 1 in 3 nitrogen species on its molecule.
Traditionally, transfection reagents must remain in contact with cells for a defined period of time, after which additional medium is added or the medium is replaced to help minimize toxic effects of the reagent. Several cho and hek293 cell lines have been engineered to further improve yield of production, notably by enabling them to grow at higher density in defined cell culture media. All available information is shown in the detailed view. Altogen transfection kits have been developed for use with both dna and sirna transfection, optimized for specific cell type in vitro or targeted to a certain tissue in vivo, and are fully compatible with serum without lowering transfection efficiency or reducing cell viability. Antibody quantities 7 days post transfection expressed by hek293 and proprietary hekinv. Atcc launches mouse cell line authentication service. Pulsin powerful delivery reagent polyplustransfection. Here, we apply four circrna identification algorithms to heuristically characterize the expression landscape of circrnas across human cancer cell lines from ccle polyaenriched.
Rphycoerythrin 1 g is complexed with 4 l of pulsin for 15 min and added to nih3t3 cells in a 24well plate. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many other cell lines. Diverging mrna and protein networks in activated microglia. Live cells are observed by fluorescence microscopy after 16 h. Towards the generation of an asfvpa104r disc mutant and a complementary cell line a potential methodology for the production of a vaccine candidate. Located close to the university of strasbourg in eastern france, polyplustransfection is iso 9001certified since 2002 and supplies its wide range of reagents for the transfection of genes, oligonucleotides and sirna through a worldwide distributor network.
Quantitative sumo proteomics identifies pias1 substrates. The table below contains a listing of the cell lines and primary cells successfully electroporated with the neon transfection system or the predecessor instrument the microporator mp100. Transfection refers to the introduction of foreign dna into a eukaryotic cell, and can be achieved by simple uptake of a precipitate mixture of calcium phosphate and the dna to be transfected. Alengo nyamayantu phd, scientific support specialist at polyplus transfection, talks about transfection trends in genome editing. Simply select the cell type, nucleic acid, and culture format to receive a qiagen transfection protocol to print out or download in convenient pdf format. Located close to the university of strasbourg in eastern france, polyplus transfection has been iso 9001certified since 2002 and supplies its proprietary range of reagents for the.
Polyplustransfection polyplustransfection sa is a biotechnology company that develops and sells innovative solutions for the delivery of nucleic acids in research, bioproduction and therapeutics. Polyplustransfection launches latest optimised transfection. With these modified properties, tge in suspension cho and hek293 cells lines relies on a transfection reagent that is efficient at different cell densities and in different synthetic media. Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. Back serum, antibiotic, cell passagedensity, transfection efficiency. Vwr offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, dna and sirna transfection, crisprcas 9 and more. Polyplustransfection biotechnology company strasbourg. It was designed for mrna delivery and showed high efficiency on a wide variety of difficult to transfect cells including lymphoma and blood cell lines, neurons and various primary cells. Fast and efficient transfection of mouse embryonic stem cells. Cd34 cdna was cloned from kg1a cell line after total rna. Drug resistance of cell line lncap1 cells after transfection were cultured until loggrowth phase and were inoculated into 100mm cell culture dishes at the concentration of 10 4 cells per dish, using. Polyplustransfection sa is a biotechnology company that develops, markets and sells innovative solutions for the in vivo, in vitro and ex vivo delivery of nucleic acids in research, bioproduction. Maintained high cell viability allows consistent protein production, leading to the extremely high protein yields observed. Please feel free to browse the polyplus transfection database to find conditions for specific product, cell line or nucleic.
Search in the publication database with polyplus transfection reagents or for optimal transfection conditions over 4000 publications, cell lines and primary cells available. Over 4000 publications, cell lines and primary cells available. I used fugene hd for transfection and i am only getting 30% effeciency. It may also refer to other methods and cell types, although other terms are often preferred. Reliable and efficient dna and rna transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. This is often the best place to start, especially in a new cell line. Factors influencing transfection efficiency thermo. Polyplus transfection sa, the leading biotechnology company that supports the gene and cell therapy market by supplying innovative transfection solutions, today announces it will make available to the market a novel transfection reagent fectoviraav in may 2020.
However, among the five myeloma cell lines, sp20, ns1 and p3u1 were hardly. Polyplustransfection sa is a biotechnology company that develops and sells. Together both polyplustransfection and in vitro technologies look forward to providing our customers with high efficiency transfection reagents that ensure high quality. Compare cell line transfection from leading suppliers on biocompare. Peipro was from polyplus transfection illkirch, france. She provides an overview of solutions for hard to transfect cells such. Transfection guide overview of transfection methods. Invitrogen product line such as lipofectamine 2000 reagent and lipofectamine 3000 reagent were used to transfect 17 cell lines with a gfpexpressing plasmid in a 24well plate format, using 0. At 18 hours posttransfection, the cell monolayer was washed once with dulbeccos phosphatebuffered saline dpbs and switched to biompm1 serum free media biological industries. The optimal time period depends on the protein being expressed, and thus the sensitivity of the assay. Search in the publication database with polyplus transfection reagents or for transfection conditions over 4000 publications, cell lines and primary cells. The dsrna is 100 500 bp long, the cell line is kc cell from culicoides sonorensis, i. At polyplus transfection, we are proud to work with reagents that are extremely gentle to cells, maintaining an excellent cell viability and morphology throughout the whole duration of the experiments. Comparative study of polyethylenimines for transient gene expression in mammalian hek293 and cho cells.
Transfection cell database sample to insight qiagen. Fast and efficient transfection of mouse embryonic stem. Of them, some groups have stably expressed the gene coding for protein of interest in mouse fibroblast cell line, nih3t3, 14 and have used the cells as immunogen. Towards the generation of an asfvpa104r disc mutant and a. Dec 11, 2017 alengo nyamayantu phd, scientific support specialist at polyplus transfection, talks about transfection trends in genome editing. Human embryonic kidney cell line hek293t and hek293t cells stably expressing egfp. Transfection technologies market 2020 rising demand with key. Quantitative analysis of highly parallel transfection in cell microarrays. This software comprises an integrated image database management component. Altogen biosystems provides in vivo transfection reagents, over 100 preoptimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. Jun 30, 2016 reliable and efficient dna and rna transfection methods are required when studying the role of individual genes in mouse pluripotent stem cells. After that, pcmv6neocd31 and mocktransfected and also untransfected nih3t3 cells were. The table below lists the range of recommended reagent amounts and volumes to use to transfect cells in various tissue culture formats.
Search in the publication database with polyplus transfection reagents or for transfection conditions over 4000 publications, cell lines and primary cells available. Polyplustransfection developed a polymerbased transfection reagent jetmessenger. Here, the authors use quantitative proteomics to identify potential pias1 substrates in human cells and elucidate the biological. Transfection reagents are highly efficient for dna and sirna transfection in vivo and in vitro. Cloned genes can be transfected into cells for biochemical characterization, mutational analyses, investigation of the effects of gene expression on cell growth, investigation of gene regulatory elements, and to produce a specific protein.
For jetprime polyplustransfection, new york, ny, usa transfection, cell cultures were prepared as. Typically, 24 to 48 hours between transfection and activity assay is sufficient. Hekinv is an optimized host cell line for tge processes generated by a directed evolution approach resulting in a 3 fold increase in productivity in comparison to the parental host cell line. Systematic lot management and release testing is performed for each lot produced. Search for publications in the transfection database with polyplustransfection reagents or for transfection conditions. Located close to the university of strasbourg in eastern france, polyplustransfection has been iso 9001certified since 2002 and supplies its proprietary range of reagents for the. Polyplus has designed fectopro to deliver very high protein yields in cho and hek cell line systems, the current industry standard systems and most commonly used by bioproduction scientists. The hek293ft cell line was selected as a standard permissive cell line for its high transfection rate 20, 24.
Comprehensive characterization of circular rnas in human. Altogen cro offers in vivo rnai services, tumor xenograft models, toxicology testing, stable cell line generation, and. The glial cell culture was maintained for 2025 days in dulbeccos. Lncap1 cells were transfected with overexpression plasmid by using the liposome transfection kit interferin polyplus transfection, usa. Polyplustransfection launches a new transfection reagent. Polyplus transfection launches the next generation. A new partnership with polyplus transfection sa in vitro. For any technical inquiries, get in touch with our friendly team of phd scientific support specialists.
Polyplus transfection launches latest optimised transfection reagent fectopro. Mirus is the leading manufacturer of transfection reagents for chemical andor electroporation delivery of nucleic acids to eukaryotic cells. Trends in transfection polyplustransfection youtube. Polyplustransfection sa is a biotechnology company that develops and sells innovative solutions for the delivery of nucleic acids. Aug 26, 2019 human cancer cell lines are fundamental models for cancer research and therapeutic strategy development. Transfection the delivery of dna or rna into eukaryotic cells is a powerful tool used to study and control gene expression. However, these cells usually grow in tight clusters and are therefore more difficult to transfect than many. However, there is no characterization of circular rnas circrnas in a large number of cancer cell lines.